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商品編號:16942

KK4824 KAPA KAPA 文庫定量試劑盒 (Illumina/通用型)

品牌:Roche-KAPA
規格:0
參考價格:6650
剩余數量:00門店價:¥0
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代理產品

品牌:KAPA,~~~~~~~~~~ 規格:~~~~~~~~~~ 價格:6650元~~~~~~~~~~~~~產品詳情請參閱KAPA官網,鏈接:https://www.kapabiosystems.com/

KAPA Library Quantification Kits contain all the reagents needed for the accurate, reliable and reproducible qPCR-based quantification of NGS libraries prior to pooling for capture or flow cell amplification. Kits contain KAPA SYBR® FAST qPCR Master Mix, optimized for high-performance SYBR Green I-based qPCR. The engineered KAPA SYBR FAST DNA Polymerase contained in the Master Mix amplifies GC- and AT-rich DNA fragments of different lengths with similar efficiency, making it the only qPCR Master Mix capable of accurate qPCR-based quantification of NGS libraries.* The pre-diluted set of DNA Standards contained in each kit is carefully quality-controlled to ensure lot-to-lot consistency and avoid data drift over time. Optimized Primer Premixes ensure optimal PCR efficiency.

Kits with DNA Standards and Primer Premixes for Illumina® and Ion Torrent™ sequencers are available. Please reference the Instrument Compatibility Chart for guidance on compatible platforms.

NEW! Improve experimental designs with our Technical Guide for Illumina Platforms


*Data on file.

For Research Use Only. Not for use in diagnostic procedures.

Product Highlights

Accurately quantify PCR-competent sequencing templates

  • qPCR only “counts” those library molecules that can be sequenced
  • More consistent cluster densities or template-to-bead ratios enable optimal utilization of sequencing resources
Equimolar pooling of indexed libraries. Eleven indexed Illumina TruSeq libraries were quantified by qPCR using the KAPA Library Quantification Kit, and then combined to achieve equal final concentrations in two separate pools for multiplexed sequencing on different flow-cell lanes. The eleven libraries ranged ~11-fold in concentration from 0.67 pM to 7.65 pM, while representation of each index varied between 90% and 127% of expected assigned reads per lane. Data on file.

Improve pooling for multiplexed sequencing

  • Library concentrations determined by qPCR are more reliable compared to other methods*
  • The engineered KAPA SYBR FAST qPCR Master Mix enables accurate pooling of libraries with extreme GC-contents or longer inserts*
Nine human DNA libraries and two microbial DNA libraries (Rhodococcus sp. ~70% GC and Staphylococcus sp. ~35% GC) were used to compare quantification results obtained with distinct lots (”Lot 1” and “Lot 2”), and distinct sets of reagents from the same lot (”Set 1” and “Set 2”) of KAPA Library Quantification Kits for Illumina platforms. Data on file.

Eliminate drift with carefully quality-controlled DNA Standards

  • Prediluted DNA Standards eliminate quantification errors associated with preparation of standard curves using in-house standards*
  • KAPA DNA Standards undergo strict quality control to ensure lot-to-lot consistency and eliminate data drift over time
KAPA Library Quantification methods (96- and 384-well) format are available for the Sciclone NGS and NGSx workstation (PerkinElmer) and other instruments used in high-throughput NGS sample preparation pipelines.

Improve throughput with automation

  • Library quantification assay is compatible with 96- and 384-well format
  • Library dilution, reaction setup and data analysis can be automated for HTP pipelines
  • Learn More about KAPA Automated Solutions

*Data on file.

~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ 杭州開泰生物技術有限公司上是一家創辦于2006年的創新型高科技企業,自公司成立以來一直專注于為生命科學和生物技術領域的客戶提供優質的產品和技術服務。公司是Tiangen,CST,KAPA,科晶和江蘇MRC的浙江一級代理。同時公司擁有自主研發的漢泰基因品牌高通量測序產品,并提供各種科研技術服務。如需訂購,請在線聯系QQ或者致電:0571-88169527.

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